FITC 抗体 (Alkaline Phosphatase (AP))
Quick Overview for FITC 抗体 (Alkaline Phosphatase (AP)) (ABIN401116)
抗原
适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Affinity Chromatography
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免疫原
- Fluorescein conjugated to Goat IgG
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亚型
- IgG
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anti-Fluorescein Isothiocyanate (FITC) antibody
FITC ELISA, WB, IHC, FACS 宿主: 绵羊 Polyclonal unconjugated
anti-Fluorescein Isothiocyanate (FITC) antibodyFITC WB 宿主: 兔 Polyclonal unconjugated
anti-Fluorescein Isothiocyanate (FITC) antibodyFITC 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Fluorescein Isothiocyanate (FITC) antibody (HRP)FITC ELISA, WB, IHC 宿主: 绵羊 Polyclonal HRP
anti-Fluorescein Isothiocyanate (FITC) antibody (Alkaline Phosphatase (AP))FITC ELISA, WB, IHC 宿主: 绵羊 Polyclonal Alkaline Phosphatase (AP)
anti-Fluorescein Isothiocyanate (FITC) antibody (Biotin)FITC ELISA, WB, IHC, FACS 宿主: 绵羊 Polyclonal Biotin
anti-Fluorescein Isothiocyanate (FITC) antibody (PE)FITC ELISA, FACS 宿主: 绵羊 Polyclonal PE
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应用备注
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ELISA: 1/2,000 - 1/10,000. Western Blot: 1/500 - 1/2,500. Immunohistochemistry: 1/200 - 1/: 1,000. Immunodiffusion.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.005 M Magnesium Chloride, 0.0005 M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0 containing 10 mg/mL BSA (IgG and Protease Free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- DO NOT FREEZE!!
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C.
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- FITC (Fluorescein Isothiocyanate (FITC))
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别名
- FITC
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物质类
- Chemical
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背景
- Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is? pH sensitive and is significantly reduced below? pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.Synonyms: Fluorescein Isothiocyanate
抗原
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